15th Conference on the Cell & Molecular Biology of Chlamydomonas

				June 05 (Tuesday)

14:00-20:00				Registration
16:30				Maria Mittag, Welcome

16:45				Keynote Lecture

				Introductory remarks: George Witman
				Bill Snell (University of Texas Southwestern Medical Center, Dallas, USA) "Cilium-generated signaling, flagellar length control, and gamete fusion in Chlamydomonas"

18:15-20:00				Reception (snacks and drinks)


				June 06 (Wednesday)

Session 1				Photosynthesis Chair: Yuichiro Takahashi (Okayama University, Japan)

08:30-08:45				Introduction/Accumulation of pigments, quinones and chlorophyll-binding polypeptides during light-induced greening of dark-grown yellow y-1 cells
08:45-09:00				Joris Snellenburg (VU University Amsterdam, The Netherlands): The state transition of Chlamydomonas reinhardtii studied at 77K using global and target analysis of time-resolved fluorescence spectroscopy data
09:00-09:15				Dimitris Petroutsos (University of Münster, Germany): Calcium dependent regulation of cyclic photosynthetic electron transfer via a CAS, ANR1 and PGRL1 complex
09:15-09:30				Ryutaro Tokutsu (National Institute for Basic Biology, Okazaki, Japan): Formation of PSII-LHCII-LHCSR super complex under NPQ inducible conditions
09:30-09:45				Paola Ferrante (Casaccia Research Center Rome, Italy): The LHCBM1 and LHCBM2/7 polypeptides, components of the major LHCII complex, have distinct functional roles in the photosynthetic antenna system of Chlamydomonas reinhardtii
09:45-10:00				Martin Lohr (Johannes Gutenberg University Mainz, Germany): Exploring the function of loroxanthin in light-harvesting complex II of Chlamydomonas reinhardtii and other green algae
Subsession				Chlamydomonadales/Volvocales
10:00-10:15				Ryo Matsuzaki (University of Tokyo, Japan): Taxonomy of Chloromonas sp. EbCl-8 (Volvocales, Chlorophyceae), based on multigene phylogeny, TEM and nuclear ribosomal DNA internal transcribed spacer 2
10:15-10:30				Takashi Hamaji (Kyoto University, Japan): Sequencing the mating type locus of the isogamous colonial Gonium pectorale

10:30-11:00				Coffee Break


Session 2				Basal bodies and transition zones Chair: Susan Dutcher (Washington University, St. Louis, USA)

11:00-11:15				Introduction/Identification and characterization of the cnc1 mutant with an altered ciliary necklace
11:15-11:30				Takashi Ishikawa (Paul Scherrer Institute, Villigen PSI, Switzerland): 3D arrangement of axonemal dyneins and regulatory proteins revealed by cryo-electron tomography
11:30-11:45				Joshua Alper (MPI of Molecular Cell Biology and Genetics, Dresden, Germany): In vitro gliding assays indicate that Chlamydomonas dynein moves microtubules polymerized from Chlamydomonas axonemal tubulin faster than those polymerized from porcine brain Tubulin
11:45-12:00				Prachee Avasthi (UCSF, San Francisco, USA): Localization of overlapping monomeric, filamentous, and phosphorylated actin populations in vegetative Chlamydomonas cells
12:00-12:15				Carolyn Silflow (University of Minnesota, St. Paul, USA): The Chlamydomonas Uni1 protein localizes to basal bodies and confers an increased capacity for flagellar assembly
12:15-12:30				Dennis Diener (Yale University, New Haven, USA): Isolation of flagellar transition zones

12:30-14:00				Lunch Buffet

14:00-16:00				Posters, Part A (Sessions 1 including the subsession, 2, 3 and 10)/Coffee


Session 3				Circadian rhythm, cell cycle and development Chair: Jim Umen (Donald Danforth Plant Science Center, St. Louis, USA)

16:00-16:15				Introduction/The population genetics of mt: resolving the divergence paradox between Chlamydomonas and Volvox mating locus genes
16:15-16:30				Yoshiki Nishimura (Kyoto University, Japan): Gsp1 triggers a sexual developmental program including the cytoplasmic inheritance inChlamydomonas reinhardtii
16:30-16:45				Takuya Matsuo (Nagoya University, Japan) Characterization of the Chlamydomonas circadian clock protein ROC15
16:45-17:00				Volker Wagner (Friedrich Schiller University Jena, Germany): The role of a protein disulfide isomerase in the circadian clock of Chlamydomonas reinhardtii
17:00-17:15				Jens Boesger (Friedrich Schiller University Jena, Germany) HEAT SHOCK FACTOR1 is involved in temperature-dependent regulation of the clock-relevant CASEIN KINASE1 in Chlamydomonas reinhardtii
17:15-17:30				Cristina Lopez-Paz (Donald Danforth Plant Science Center, St Louis, USA): TNY1, an hnRNP like protein that functions as a cell cycle repressor in the RB size checkpoint pathway
17:30-17:45				Kateřina Bišová (ASCR, Třeboň, Czech Republic) Coordination of cell cycle progression and DNA damage response by wer (WEE1 regulation) mutants in Chlamydomonas reinhardtii
17:45-18:00				Bradley Olson (Kansas State University, Manhattan, USA): Evolution of cell cycle control in the volvocine algae: from unicellular Chlamydomonas to colonial multicellular Gonium

18:00-19:45				Dinner Buffet

				Workshop on knock-out strategies of Chlamydomonas genes Chair: Olivier Vallon (CNRS, Paris, France)

19:45-20:00				Introduction/Towards a library of Chlamydomonas flanking sequence tags
20:00-20:15				Karl F. Lechtreck (University of Georgia, Athens, USA): Promoter-less aph7" as a tool for insertional mutagenesis and promoter-tagging in Chlamydomonas reinhardtii
20:15-20:30				Martin Jonikas (Carnegie Institution for Science, Stanford, USA): Genome-wide mutagenesis and phenotyping tools to transform our understanding of photosynthesis
20:30-20:45				Irina Sizova (Humboldt-University, Berlin,Germany): Targeted knockout of the nonselectable ChRI gene in Chlamydomonas cells by using zinc-finger nucleases
20:45-21:00				Susan Dutcher (Washington University, St. Louis, USA): Whole genome sequencing to identify mutations in the Chlamydomonas genome
21:00-21:15				Discussion


				June 07 (Thursday)

Session 4				-omics and systems biology Chair: Michael Schroda (MPI MPP, Potsdam-Golm, Germany)

08:30-08:45				Introduction/Timo Mühlhaus (MPI MPP, Golm, Germany): IOMIQS (Integration of mass spectrometry identification and quantification software) features automated evaluation of shotgun proteomics data and database-based intelligent data management
08:45-09:00				Tabea Mettler (MPI MPP, Potsdam-Golm, Germany): Findings of the GoFORSYS systems biology project of Chlamydomonas reinhardtii: How the Calvin-Benson cycle and end product pathways adapt to an increase in light intensity and its implication for growth
09:00-09:15				Stéphane Lemaire (University Pierre and Marie Curie, Paris, France): Glutathionylation in the photosynthetic model-organism Chlamydomonas reinhardtii: a proteomic survey
09:15-09:30				Kourosh Salehi-Ashtiani (New York University, USA): Evolutionary analyses of Chlamydomonas reinhardtii metabolic network suggest coupled co-evolution over long evolutionary distances
09:30-09:45				Ricarda Höhner (University of Münster, Germany): Quantitative proteomics and protein network analysis reveal that iron-deficiency responses are driven by iron-availability and the metabolic status in Chlamydomonas reinhardtii
09:45-10:00				Jonathan Flowers (New York University, USA): A catalog of genetic variation in wild isolates of Chlamydomonas reinhardtii
10:00-10:15				Simon Alfred (University of Toronto, Canada): HTS identification of Chlamydomonas reinhardtii bioactive molecules that affect fitness, photosynthesis and phototaxis
10:15-10:30				Ian Blaby (University of California, Los Angeles, USA): A systems analysis of a Chlamydomonas starch less mutant

10:30-11:00				Coffee Break


Session 5				Light perception and responses Chair: Hironao Kataoka (Botanical Gardens, Tohoku University, Japan)

11:00-11:15				Introduction
11:15-11:30				Meike Luck (Humboldt University Berlin, Germany): A bimodal photo-convertable UV-Rhodopsin of Chlamydomonas reinhardtii
11:30-11:45				Franziska Schneider (Humboldt University Berlin, Germany): Ion selectivity and gating in Channelrhodopsins
11:45-12:00				Tilman Kottke (University Bielefeld, Germany): Characterization of a cryptochrome photoreceptor which is active in Chlamydomonas
12:00-12:15				Georg Kreimer (Friedrich Alexander University Erlangen, Germany): Phototropin, a novel player in eyespot development and control of phototaxis in Chlamydomonas reinhardtii
12:15-12:30				Mark Thompson (University of Arizona, Tucson, USA): Order of assembly of eyespot proteins after cell division

12:30-14:00				Lunch Buffet

14:00-16:00				Posters, Part B (Sessions 4, 5, 6 and 7)/Coffee


Session 6				Adaptation and stress response Chair: Claire Remacle (University of Liège, Belgium)

16:00-16:15				Introduction/Characterization of an internal type-II NADH dehydrogenase from Chlamydomonas reinhardtii mitochondria
16:15-16:30				Lior Atiya (Ben-Gurion University of the Negev, Beer Sheva, Israel): CrBsd2 is a redox-regulated chaperone that prevents the formation of non-native disulfide bridges during synthesis of the large subunit of Rubisco
16:30-16:45				Krishna Niyogi (University of California, Berkeley, USA): A regulator of singlet oxygen response in Chlamydomonas reinhardtii
16:45-17:00				Donald Weeks (University of Nebraska, Lincoln, USA): Massive changes in gene expression associated with induction of the CO2 concentrating mechanism in Chlamydomonas reinhardtii
17:00-17:15				Dorothea Hemme/Daniel Veyel (MPI MPP Potsdam-Golm, Germany): Stress Dynamics: How Chlamydomonas reinhardtii suffers and recovers from heat stress
17:15-17:30				Carola Päpke (MPI MPP Potsdam-Golm, Germany): Regulation of respiration during low oxygen availability in Chlamydomonas reinhardtii
17:30-17:45				Karin Komsic-Buchmann (University of Cologne, Germany): The SEC6 protein is required for function of the contractile vacuole in Chlamydomonas reinhardtii
17:45-18:00				Maria Esther Pérez-Pérez (CSIC-University Sevilla, Spain): Photo-oxidative damage triggers autophagy in Chlamydomonas

18:00-19:45				Dinner Buffet

19:45-21:45				Special lectures:

				Introductory remarks: Ralph Bock
				Nathan Nelson (Tel Aviv University, Israel)
				"Structure, function and evolution of Photosystem I"
				Introductory remarks: Maria Mittag
				Angela Falciatore (CNRS-UPMC, Paris, France)
				"Unveiling the molecular mechanisms of light responses in marine diatoms"


				June 08 (Friday)

Session 7				Biofuels Chair: Anja Hemschemeier (University Bochum, Germany)

08:30-08:45				Introduction
08:45-09:00				Jan Mussgnug (University Bielefeld, Germany): New insights into light energy conversion for product generation with Chlamydomonas reinhardtii
09:00-09:15				Gilles Peltier (CNRS-CEA-Aix Marseille University, Saint-Paul-lez-Durance, France): Limiting factors of hydrogen photoproduction as studied in Chlamydomonas mutants affected in cyclic electron flow around photosystem I
09:15-09:30				Alexandra Dubini (NREL, Golden, USA): Deconvolution of the ferredoxin-centered metabolic network in Chlamydomonas reinhardtii and its relationship to hydrogen production pathway
09:30-09:45				Anja Günther (University Leipzig, Germany): Genetic modification of the photorespiratory pathway in Chlamydomonas reinhardtii for bioenergetic application
09:45-10:00				Pierre Cardol (University of Liège, Belgium): Light-induced photosynthetic electron transfer upon anaerobiosis in Chlamydomonas: kinetics, electron sinks and setup of a fluorescence screen to identify new players
10:00-10:15				Qiang Hu (Arizona State University, Mesa, USA): Biogenesis and function of lipid bodies in microalgae and implications for algal biofuels production
10:15-10:30				Yonghua Li-Beisson (CEA-CNRS-Aix-Marseille University, CEA Cadarache, France): Deciphering triacylglycerol accumulation in Chlamydomonas reinhardtii using a proteomic approach

10:30-11:00				Coffee Break


Session 8				Frontiers in gene expression Chair: Laurence Maréchal-Drouard (University of Strasbourg, France)

11:00-11:15				Introduction/Differential import of tRNAs into Chlamydomonas mitochondria: a question of evolutionary selective pressure rather than a dynamic process
11:15-11:30				Lutz Wobbe (Bielefeld University, Germany): Molecular function of a mitochondrial DNA-binding protein from C. reinhardtii
11:30-11:45				Daniela Strenkert (MPI MPP Potsdam-Golm, Germany): Activation of downstream promoters by the HSP70A promoter in transgenes is not mediated by an open chromatin structure per se, but requires promoter occupancy by heat shock factor 1
11:45-12:00				Heriberto Cerutti (University of Nebraska, Lincoln, USA): Identification of argonaute-associated small RNAs and their target transcripts in Chlamydomonas reinhardtii
12:00-12:15				Simon Prochnik (DOE-Joint Genome Institute, Walnut Creek, USA): Chlamydomonas v.5 genome: Dramatic improvements in assembly and annotations
12:15-12:30				Ru Zhang (Carnegie Institution for Science, Stanford, USA): A novel, high through put genomic tool to comprehensively identify genes essential for photosynthesis and growth under high light in Chlamydomonas

12:30				Lunch Package

13:15				Departure for local excursion Boat tour on the Spree

20:00				Banquet


				June 09 (Saturday)

Session 9				Metabolism Chair: Aurora Galván (University of Córdoba, Spain)

08:30-08:45				Introduction/Identification of genes for nitrate signalling in Chlamydomonas by analysis of insertional mutants
08:45-09:00				Su-Chiung Fang (Academia Sinica, Tainan, Taiwan): SMT15 connects sulfur-related cellular response to cell cycle control in Chlamydomonas
09:00-09:15				Erik Hom (Harvard University, Cambridge, USA): A novel mutualism between Saccharomyces cerevisiae and Chlamydomonas reinhardtii
09:15-09:30				Elena Chekunova (Saint Petersburg University, Russia): New genes involved in the transcriptional control of Mg-chelatase activity in Chlamydomonas reinhardtii
09:30-09:45				Simone Zäuner (University of Bonn, Germany): A cytochrome b5-containing plastid-located fatty acid desaturase from Chlamydomonas reinhardtii
09:45-10:00				Severin Sasso (Friedrich Schiller University Jena, Germany): A genomic perspective on secondary metabolism in Chlamydomonas reinhardtii and other sequenced algae
10:00-10:15				Elena Ermilova (Saint Petersburg University, Russia): PII signal transduction protein in Chlamydomonas reinhardtii: characterization and putative role
10:15-10:30				Flor Martínez (University Autónoma de Madrid, Spain): Molecular dissection of the N-Glycosylation biosynthetic pathway in the green alga Chlamydomonas reinhardtii

10:30-11:00				Coffee Break


Session 10				Flagellar transport and assembly Chair: George Witman (University of Massachusetts, Worcester, USA)

11:00-11:15				Introduction/Karl F. Lechtreck (University of Georgia, Athens, USA): The Chlamydomonas reinhardtii BBSome functions as a cargo adaptor for retrograde IFT
11:15-11:30				Hongmin Qin (Texas A&M University, College Station, USA): The RABL5 homolog IFT22 regulates the cellular pool size and the amount of IFT particles partitioned to the flagellar compartment in Chlamydomonas reinhardtii
11:30-11:45				Stephen King (University of Connecticut, Mansfield, USA): Association of Lis1 with outer arm dynein is modulated in response to alterations in flagellar motility
11:45-12:00				Paul Lefebvre (University of Minnesota, St. Paul, USA): A new gene controlling flagellar length, LF5, encodes a flagellar form of the CDKL5 kinase
12:00-12:15				Glen Wheeler (Marine Biological Association/Plymouth Marine Laboratory,Plymouth, UK): Rapid Ca²⁺ elevations in Chlamydomonas flagella associated with gliding motility
12:15-12:30				Winfield Sale (Emory University School of Medicine, Atlanta, USA): Analysis of temporary dikaryons reveal that transport of I1 dynein in the flagellum requires the intermediate chain subunit IC140 and Ida3p

12:30-14:00				Lunch Buffet

14:00-16:00				Posters, Part C (Sessions 8, 9 and 11)/Coffee


Session 11				Chloroplast function and biogenesis Chair: Michel Goldschmidt-Clermont (University of Geneva, Switzerland)

16:00-16:15				Introduction
16:15-16:30				Jessica Jacobs (University Bochum, Germany): Evidence for a chloroplast spliceosome involved in RNA trans-splicing
16:30-16:45				Kevin Redding (Arizona State University, USA): Degradation of Photosystem I by a coalition of proteases
16:45-17:00				Alexandra-Viola Bohne (Ludwig Maximillians University, Planegg-Martinsried, Germany): Reciprocal regulation of protein synthesis and carbon metabolism for thylakoid membrane biogenesis?
17:00-17:15				Shin-ichiro Ozawa (UMR CNRS/UPMC Paris, France): RDH1, a nucleus-encoded factor required for atpH transcript stabilisation and atpI mRNA translation in the chloroplast of Chlamydomonas reinhardtii
17:15-17:30				Henning Kirst (University of California, Berkeley, USA): Assembly of the light-harvesting chlorophyll antenna in the green alga Chlamydomonas reinhardtii requires expression of the TLA2-CpFTSY gene
17:30-17:45				Catherine de Vitry (CNRS/UPMC UMR, IBPC Paris, France): FtsH as a major house-keeping protease in the thylakoid membrane of Chlamydomonas
17:45-18:00				Silvia Ramundo (University of Geneva, Switzerland): Conditional inactivation of the two essential plastid genes rpoA and rps12 through vitamin-mediated control of chloroplast gene expression in Chlamydomonas

18:00-19:45				Dinner Buffet

19:45-21:15				"Fossil" lecture:

				Introductory remarks: Sabeeha Merchant
				Arthur Grossman (Carnegie Institution, Stanford University, USA) "From light to dark with Chlamydomonas"

				Poster awards


				June 10 (Sunday)

08:30				Departure after breakfast

Detailed Schedule